Co-activation of GR and NF-kB leads to extensive rearrangemnet of their binding sites and target genes. Homo sapiens

Global identification of activated GR and p65 binding sites and target genes using ChIP-seq in HeLa B2 cells. Overall design: generation genome-wide chromatin state-maps of GR, p65 and RNAPII in HeLa B2 cells under conditions 1) DMSO (control); 2) TA 1µM 4hr; 3) TNFα 10ng/ml ; 4) TA 1µM 4hr at at third hour 10ng/ml TNF α was added

在HeLa B2细胞中利用染色质免疫共沉淀测序（ChIP-seq）全局鉴定激活的糖皮质激素受体（GR）与p65的结合位点及其靶基因。
实验设计：在HeLa B2细胞中，针对以下四种实验条件绘制GR、p65及RNA聚合酶II（RNAPII）的全基因组染色质状态图谱：1）二甲基亚砜（DMSO，对照组）；2）以1μM TA处理4小时；3）以10ng/ml肿瘤坏死因子α（TNFα）处理；4）以1μM TA处理4小时，并在处理的第3小时加入10ng/ml TNFα。