Effect of DES-treated Ept congenic rat lines on gene expression in the anterior pituitary gland.. Rattus norvegicus

Anterior pituitary glands were isolated from 21 week old male rats treated for 12 weeks with the synthetic estrogen diethylstilbestrol (DES). Three biological replicates were prepared for each of 4 congenic lines: Ept1, Ept2, Ept6, and Ept9. Congenic rat lines were constructed by introgressing COP alleles onto an ACI background within a given Ept locus. Expression profiles were determined using Affymetrix Rat Genome 230 v. 2.0 arrays. Comparison of congenic rats with the ACI and COP parental strains (see series GSE4028) will allow identification of genes whose expression is influenced by an estrogen-induced pituitary tumor (Ept) QTL. Keywords: Estrogen Response Overall design: 12 samples from anterior pituitary gland were analyzed. For each of 4 congenic rat lines (Ept1, Ept2, Ept6, Ept9) there were 3 biological replicates of DES-treated male animals. Congenic animals were constructed by introgressing COP alleles onto an ACI background. This was done within the linkage interval for a given Ept locus. These congenic samples can be compared to the ACI and COP parental strains (see GSE4028) to determine expression differences due to a particular chromosomal region, or QTL.

本研究从经合成雌激素己烯雌酚（diethylstilbestrol, DES）处理12周的21周龄雄性大鼠体内分离垂体前叶组织。针对Ept1、Ept2、Ept6及Ept9这4个同类系（congenic lines），每个品系均制备3份生物学重复样本。同类系大鼠的构建方式为将COP等位基因回交导入至ACI遗传背景中，且该导入过程限定于特定的雌激素诱导垂体瘤（Estrogen-induced pituitary tumor, Ept）位点的连锁区间内。本研究采用Affymetrix大鼠基因组230 v2.0芯片检测样本的基因表达谱。将同类系大鼠与ACI、COP亲本品系（详见数据集系列GSE4028）进行比较，可鉴定出受雌激素诱导垂体瘤（Ept）数量性状位点（quantitative trait locus, QTL）调控表达的基因。
关键词：雌激素应答
实验整体设计：
本研究共分析12份垂体前叶组织样本。针对4个同类系大鼠（Ept1、Ept2、Ept6、Ept9），每个品系均包含3份经DES处理的雄性动物生物学重复样本。同类系动物的构建方式为将COP等位基因导入ACI遗传背景中，且该导入过程限定于特定Ept位点的连锁区间内。通过将这些同类系样本与ACI、COP亲本品系（详见GSE4028）进行比对，可明确特定染色体区域（即QTL）所导致的基因表达差异。