Aspergillus oryzae LC-MSMS - Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae

We previously identified the bHLH transcription factor SclR and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast extract liquid medium. Therefore, this study presents a comparative assessment at the proteome level of the intracellular differences between an sclR-disrupted strain and a control strain using isobaric tandem mass tag (TMT) labeling for quantification.

本课题组此前已鉴定出bHLH转录因子（bHLH transcription factor）SclR，并发现SclR功能缺失会引发显著表型变化，例如在糊精-蛋白胨-酵母膏液体培养基中出现快速蛋白质降解与细胞裂解现象。为此，本研究采用同量异位素串联质量标签（isobaric tandem mass tag, TMT）标记定量技术，在蛋白质组层面对sclR敲除菌株与对照菌株的细胞内蛋白质组差异进行了比较分析。