MicroRNA Expression in Bone Marrow-Derived Human MSCs. MicroRNA Expression in Bone Marrow-Derived Human MSCs

Six commercially available MSC lines were expanded from passage 3 to 7 and their miRNA expression was evaluated using microarray technology. Statistical analyses of our data revealed that 71 miRNAs out of 939 examined were expressed by this set of MSC lines at all passages and the expression of 11 miRNAs were significantly different between passage 3 and 7. Overall design: Randomized Block Design - Repeated Measures Experiment: MSCs from 8 different donors at 3 passages (passages 3, 5, & 7) with technical replicates at each passage

我们将6株市售间充质干细胞（Mesenchymal Stem Cells, MSC）系从第3代扩增至第7代，并采用微阵列（microarray）技术检测其微小RNA（microRNA, miRNA）的表达情况。对所得数据进行统计学分析后发现，在939个被检测的微小RNA中，有71个在该组MSC系的所有传代阶段均有表达；另有11个微小RNA的表达水平在第3代与第7代之间存在显著差异。实验整体设计：随机区组设计-重复测量实验：使用来自8名不同供体的间充质干细胞，分别在3个传代阶段（第3、5、7代）开展实验，且每个传代阶段均设置技术重复。