Comparison of CRISPR-Cas9 genome-edited JEG-3 TAZ/WWTR1 knockout and wild type choriocarcinoma cells

The transcriptional co-activator TAZ/WWTR1 plays a central role in the Hippo signaling pathway and acts as crucial mediator in maintaining organ size and tissue homeostasis. Changes in its activity can lead to diseases, including cancer, due to uncontrolled cell growth or aberrant cellular behavior. The aim of this study was to identify gene signatures that are specifically regulated by TAZ in human trophoblast cells. This was achieved by comparing JEG-3 wild-type and TAZ knockout choriocarcinoma cells, generated with CRISPR-Cas9 technology. The JEG-3 TAZ wild-type and knockout cell lines were generated using CRISPR-Cas9 genome-editing, resulting in two wild-type and two TAZ knockout lines. Next-Generation Sequencing was employed to analyze the gene expression differences across 8 samples, comprising the two wild-type cell lines and the two knockout cell lines, each measured in duplicates.

转录共激活因子TAZ/WWTR1在Hippo信号通路（Hippo signaling pathway）中发挥核心作用，是维持器官大小与组织稳态的关键介质。其活性异常可因细胞生长失控或细胞行为畸变引发包括癌症在内的多种疾病。本研究旨在筛选人类滋养层细胞中受TAZ特异性调控的基因特征（gene signatures）。为此，本研究通过比较经CRISPR-Cas9技术构建的JEG-3野生型与TAZ基因敲除绒毛膜癌细胞系开展实验。本研究利用CRISPR-Cas9基因组编辑技术构建了JEG-3 TAZ野生型与敲除细胞系，最终获得2株野生型细胞系与2株TAZ基因敲除细胞系。随后采用下一代测序技术（Next-Generation Sequencing）对8份样本的基因表达差异进行分析，该8份样本涵盖2株野生型细胞系与2株敲除细胞系，每组均设置2次重复检测。