Drosophila melanogaster sex transformed larval gonads. Drosophila melanogaster

Drosophila sex determination and gonadal development is regulated at both the pre-mRNA splicing level, by the RNA-binding protein encoded by transformer (tra), and at the transcriptional level by the DNA-binding protein encoded by doublesex (dsx) (https://doi.org/10.1016/S0074-7696(02)19010-3). Wild type chromosomal males (XY) do not expressed tra resulting in expression the male isoform of dsx (dsxM) and develop testes. XX females null for tra (tra-) or expressing dsxM have ovaries transformed into testes. Wild type chromosomal females (XX) express functional tra resulting in expression of the female form of dsx (dsxF). XY males expressing tra from a transgene (traF) express dsxF and have testes transformed into ovaries. We were interested in capturing deep profiles of these gonads including alternative start sites and splice forms.

果蝇的性别决定与性腺发育受到双重调控，分别作用于pre-mRNA剪接（pre-mRNA splicing）与转录水平：前者由transformer（tra）基因编码的RNA结合蛋白（RNA-binding protein）介导，后者则由doublesex（dsx）基因编码的DNA结合蛋白（DNA-binding protein）调控（文献DOI：https://doi.org/10.1016/S0074-7696(02)19010-3）。野生型染色体雄性（XY）不表达tra，因此产生雄性型dsx（dsxM）并发育为睾丸。XX型雌性若发生tra功能缺失（tra⁻）或表达dsxM，则其卵巢会转化为睾丸。野生型染色体雌性（XX）可表达具有功能的tra，进而产生雌性型dsx（dsxF）。若通过转基因（traF）表达tra的XY雄性个体，则会表达dsxF，其睾丸会转化为卵巢。本数据集旨在获取上述各类性腺的深度测序图谱，涵盖可变起始位点与可变剪接亚型。