Chronic Helicobacter pylori Infection with Sydney Strain 1 and Mouse-Adapted Sydney Strain 2000

This experiment set contains the raw data for 8 arrays that were used in the genomic typing of the pre- and post-mouse H. pylori strains SS1 and SS2000. 10700 is the pre-mouse clinical isolate of SS1 and PMSS2000 is the pre-mouse clinical isolate of SS2000. gDNA from these strains were labeled and hybridized to H. pylori microarrays as described in Salama et al. {Salama et al. 2000 PNAS 97:14668-73}. In each case the test gDNA sample was labeled with Cy5 (red) and this was hybridized with Cy3 (green) labeled reference DNA of equal amount. The reference DNA consisted of equal amounts of gDNA from the two H. pylori strains used to make the H. pylori microarray, 26695 and J99. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set Computed

本实验集收录了用于幽门螺杆菌（H. pylori）菌株SS1与SS2000小鼠定植前、定植后基因组分型的8张基因芯片的原始数据。菌株10700为SS1的小鼠定植前临床分离株，PMSS2000则为SS2000的小鼠定植前临床分离株。提取自上述菌株的基因组DNA（gDNA）参照Salama等人于2000年发表在《美国国家科学院院刊》（PNAS）第97卷14668-14673页的方法进行标记，并与幽门螺杆菌基因芯片完成杂交。所有实验组均采用Cy5（红色荧光）标记待测基因组DNA样本，并与等量Cy3（绿色荧光）标记的参考基因组DNA进行杂交。参考基因组DNA由制备该幽门螺杆菌基因芯片所用的两株幽门螺杆菌菌株26695与J99的基因组DNA等比例混合制备而成。本芯片集依据共通的生物学背景进行归类，涵盖物种、肿瘤类型、生物学过程等类别。关键词：逻辑集（Logical Set）、计算型（Computed）