Agro_AD1408_from plate_20150831

Agro_AD1408_GFP-VirB11 (Kan+AS plates, Mes pH5.8). Add 400 ul of induction media to one of the induction plates (containing lawn of Agrobacterium AD1408) and wash the plate by gently rolling the liquid. I had 20ug/ml Kan to keep the cells happier. Add gold to this: 1ul of gold to 4 ul of cells and freeze.

Agro_AD1408_GFP-VirB11（含卡那霉素（Kanamycin）与乙酰丁香酮（Acetosyringone）的平板，采用MES（2-(N-吗啉代)乙磺酸）缓冲体系，pH5.8）。向其中一块铺有农杆菌AD1408菌苔的诱导平板中加入400 μL诱导培养基，通过轻柔晃动液体以冲洗平板表面。本实验添加终浓度20 μg/mL的卡那霉素以维持菌体活性状态。向该菌体悬液体系中加入金微弹：按1 μL金微弹与4 μL菌体的比例混合后进行冷冻处理。