Kolmancic-Zivin-Zorovic_E2_L-dopa_data2022.sav

The data are the results of behavioural testing and immunohistochemistry from a study on the effects of estrogen on the development of levodopa-induced dyskinesias in a hemiparkinsonian postmenopausal rat model. The animal study protocol was approved by the Republic of Slovenia's Administration for Food Safety, Veterinary Sector and Plant Protection (ref. no. U34401-7/2017/9). The rats were overiectomized at the start of the experiment. Two weeks following the 6-OHDA lesion of dopaminergic neurons in the right hemisphere and implantation of either empty (control) or estradiol implants, the animals started recieiving daily injections of either levodopa or saline (control). Animals were divided into four tratment groups: animals with estradiol implants and daily levodopa injections, animals with empty implants and daily levodopa injections, animals with estradiol implants and daily injections of saline and animals with empty implants and daily saline injections. Behavioural testing included tests to establish the level of levodopa-induced dyskinesias (LIDs), rotation tests (RT) and the forepaw adjusting step (FAS) test to assess the motor performance. Animal body weight was followed throughout the experiment and serum levels of estradiol were measured after euthanasia. Immunohistochemistry with anti-TH primary antibody was performed on frozen brain sections to establish the extent of the 6-OHDA lesion. We also evaluated the changes in expression of FosB protein in animals from different treatments.<br>

本数据集来源于一项探究雌激素对偏帕金森病样绝经后大鼠模型中左旋多巴诱发异动症（levodopa-induced dyskinesias, LIDs）发生发展影响的研究，包含行为学测试与免疫组化实验结果。本动物实验方案已获得斯洛文尼亚共和国食品安全、兽医部门与植物保护局批准（批准号：U34401-7/2017/9）。实验伊始，对大鼠施行卵巢切除术。在对大鼠右侧脑区多巴胺能神经元进行6-羟基多巴胺（6-OHDA）损毁，并植入空白（对照）或雌二醇植入物后两周，每日对大鼠分别注射左旋多巴或生理盐水（对照）。实验动物被分为四组：雌二醇植入+每日左旋多巴注射组、空白植入+每日左旋多巴注射组、雌二醇植入+每日生理盐水注射组，以及空白植入+每日生理盐水注射组。行为学测试涵盖用于评估左旋多巴诱发异动症严重程度的测试、旋转实验（rotation tests, RT）与用于评价运动功能的前肢调整步幅（forepaw adjusting step, FAS）测试。实验全程监测大鼠体重，并在安乐死后检测其血清雌二醇水平。研究人员以抗酪氨酸羟化酶（tyrosine hydroxylase, TH）一抗对冰冻脑切片开展免疫组化染色，以确定6-OHDA损毁的范围。本研究同时分析了不同处理组大鼠脑内FosB蛋白的表达变化。