A comparison of eukaryote community composition in four contrasting sediments in the Celtic Sea

As part of the NERC funded Shelf Seas Biogeochemistry program, microbial structure, diversity and abundance was compared in four contrasting sediment types, from mud to sand. Samples were collected before (March 2015) and immediately after a spring diatom bloom (May) and also again in late summer (August). These data are 16S rRNA and 18S rRNA gene sequence sets from the four sediment types, and from the three sampling time points. Relative abundance and composition was determined using Illumina MiSeq (MrDNA, TX, USA). The V1–V3 region of 16S rRNA was amplified using the PCR primers 27F (Vergin et al., 1998) and 519Rmod (Andreotti et al., 2011) using the PCR and sequencing conditions described in Tait et al. (2015). For 18S rRNA genes, the primers Euk7F and Euk570R were used.

本研究作为英国自然环境研究理事会（Natural Environment Research Council, NERC）资助的陆架海生物地球化学项目的一部分，对覆盖泥质至砂质的四种不同沉积物类型中的微生物群落结构、多样性与丰度进行了比较。样本分别于春季硅藻藻华发生前（2015年3月）、藻华发生后即刻（5月）以及夏末（8月）三个时间点采集。本数据集包含来自上述四种沉积物类型及三个采样时间点的16S rRNA与18S rRNA基因序列数据。群落相对丰度与组成通过Illumina MiSeq测序平台测定，测序工作由美国德克萨斯州的MrDNA实验室完成。16S rRNA的V1-V3高变区采用PCR引物27F（Vergin等，1998）与519Rmod（Andreotti等，2011）进行扩增，扩增及测序条件参照Tait等（2015）的研究方法。针对18S rRNA基因，实验采用引物Euk7F与Euk570R完成扩增。