Additional tables for "Quantitative catalogue of mammalian mitotic chromosome-associated RNAs"

We present comprehensive and quantitative characterizations of full-length mitotic chromosome-associated RNAs (mCARs) in three human cell lines (A549, ARPE-19, HT-1080), one monkey cell line (CV-1), and one mouse cell line (STO). This characterization was achieved through high-depth RNA sequencing. The enriched mCARs were identified using genomic DNA-based quantification of RNA-seq data, while the identification of unannotated RNAs relied on de novo assembly. Here the detail information of tables are shown below.Table S1_List of annotated mCARs in ARPE-19 ( Table S1-S1), A549 (Table S1-S2), HT-1080 (Table S1-S3), and CV-1 (Table S1-S4), STO (Table S1-S5).Table S2_List of Novel mCARs in ARPE-19 (Table S2-S1), A549 (Table S2-S2), HT-1080 (Table S2-S3), and CV-1 (Table S2-S4), STO (Table S2-S5).Table S3_The conversion factors needed to convert the measured TPM fold-change ratio to the proper enrichment factor for each cell-type. The final conversion factor is obtained by multiplying the slope of the qPCR fold-change vs TPM fold-change relationship (Supplementary Fig. 3) by 1/(Fraction retained).Table S4_The primers of the target RNAs for the qPCR in human, mouse, and monkey cells.

本研究针对3株人类细胞系（A549、ARPE-19、HT-1080）、1株猴细胞系（CV-1）及1株小鼠细胞系（STO）中的全长有丝分裂染色体结合RNA（mitotic chromosome-associated RNAs，mCARs）开展了系统性定量表征。该表征工作通过高通量RNA测序完成。富集型mCARs通过基于基因组DNA的RNA测序数据定量分析得以鉴定，而未注释RNA的鉴定则依赖从头组装（de novo assembly）。

如下为各表格的详细信息：
表S1：各细胞系中注释型mCARs列表，其中ARPE-19对应表S1-S1、A549对应表S1-S2、HT-1080对应表S1-S3、CV-1对应表S1-S4、STO对应表S1-S5。
表S2：各细胞系中新发现mCARs列表，其中ARPE-19对应表S2-S1、A549对应表S2-S2、HT-1080对应表S2-S3、CV-1对应表S2-S4、STO对应表S2-S5。
表S3：各细胞系中将实测TPM（转录本每百万）倍数变化比值转换为标准化富集因子所需的转换系数。最终转换系数通过将qPCR（实时定量聚合酶链式反应）倍数变化与TPM倍数变化的相关性斜率（补充图3）乘以1/(留存分数)得到。
表S4：人、小鼠及猴细胞中qPCR所用靶标RNA引物序列。