<b>Figure 2. </b><b>Experimental design for inoculating </b><b>α</b><b>-synuclein pre-formed fibrils in M83 mice </b><b><i>in vivo</i></b>.

(B) Brain homogenates from aSyn PFF-1 and PFF-2 inoculated mice both displayed resistance to proteinase K but exhibited variable banding patterns upon digestion, as assessed by Western Blot using an antibody for total aSyn. (C) Brain homogenates from aSyn PFF-1 and PFF-2 inoculated mice were evaluated by dotblots using an antibody with high affinity for aggregated aSyn (chBIIB054), revealing that while both fibril types exhibited significant binding, aSyn PFF-2 brain homogenates presented with a stronger signal.(B) 接种aSyn（α-synuclein）PFF-1与PFF-2的小鼠脑组织匀浆均对蛋白酶K（Proteinase K）具有抗性，但经蛋白酶K消化后，使用针对总aSyn的抗体进行蛋白质印迹法（Western Blot）检测时，呈现出不同的条带模式。(C) 使用针对聚集型aSyn（aggregated aSyn）的高亲和力抗体chBIIB054，通过斑点印迹法（Dot Blot）对接种aSyn PFF-1与PFF-2的小鼠脑组织匀浆进行检测，结果表明：尽管两种预形成纤维样本均呈现出显著的结合信号，但aSyn PFF-2组的脑组织匀浆信号更强。