IgG1 Fc-glycosylation features of IVIg before and after SA-enrichment.

Glycopeptides were registered by nanoLC-ESI-ion trap-mass spectrometry (see Figure 2). Peak heights of glycopeptide signals (proton adducts [M+4H]4+) were integrated and normalized to the total peak heights of glycopeptide signals. The average of two measurements is given.糖肽经纳米液相色谱-电喷雾电离-离子阱质谱（nanoLC-ESI-ion trap-mass spectrometry）完成检测与记录，具体实验流程详见图2。研究人员对糖肽信号峰（质子加合物[M+4H]⁴⁺）的峰高进行积分，并以所有糖肽信号的总峰高为基准完成归一化处理，最终结果给出两次独立测量的平均值。