Capturing SARS-cov in low abundant patient samples: a comparative analysis (Illumina respv NS).

In this study, we tested the efficacy of five commercial probes panels at detecting SARS-CoV-2 genome including panels from Illumina, Twist Bioscience and Arbor Bioscience. To do so, we used 19 patient nasal swab samples broken down into 5 series of 4 samples of equivalent SARS-CoV-2 viral load (cycle threshold (CT): low CT means a high viral load – CT26, CT29, CT32, CT35 and CT36+).

本研究对5款商用探针组（probe panel）检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）基因组的效能进行了评估，所涉探针组来自Illumina、Twist Bioscience及Arbor Bioscience三家厂商。为此，我们纳入19份患者鼻拭子样本，将其按SARS-CoV-2病毒载量划分为5组，每组包含4份病毒载量相当的样本：循环阈值（cycle threshold, CT）越低代表病毒载量越高，对应分组为CT26、CT29、CT32、CT35及CT36+。