Surface water 16S rRNA gene amplicons from the NSERC Canadian Lake Pulse Network. LakePulse surface water 16S rRNA gene V4 region amplicons

To survey the bacterial diversity in Canadian lakes, a fragment of the 16S rRNA gene V4 region was amplified using the universal primer set 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') from DNA sampled from the surface waters of >200 lakes (filtered between 0.22 to 100 μm).

为调查加拿大湖泊的细菌多样性，研究人员以200余个湖泊表层水体（经0.22 μm至100 μm孔径滤膜过滤）中提取的DNA为模板，使用通用引物对515F（5'-GTGCCAGCMGCCGCGGTAA-3'）与806R（5'-GGACTACHVGGGTWTCTAAT-3'），扩增了16S核糖体RNA（16S rRNA）基因的V4区片段。