Neuronal cell labelled with anti PSD95 and homer antibodies - sample image for software testing of Easy SODA

Hippocampal neurons in culture seeded on glass coverslip. This 16 bits confocal picture of hippocampal neuron in culture has been taken at 63x (1024x1024 pixels - pixel size 92,45 nm) in sequential mode with two channels : one dedicated to the PSD95 protein, and the other one to Homer protein. Those protein are expressed at glutamatergic post-synaptic sites and can be found close within Post Synaptic Density. This is a good sample to test colocalization and association analysis. The homer channel can be used both to infere neuronal shape thanks to hk-means segmentation (http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/), or to detect clusters thanks to wavelet segmentation (http://icy.bioimageanalysis.org/protocol/rainbow-spot-detector-scale-finder/). This sample image can be used with Easy SODA protocol (Standard Object Distance Analysis) on Icy software (http://icy.bioimageanalysis.org/protocol/easy-soda-2-colors-1-image/) to detect association between PSD95 and homer clusters.

本数据集为接种于玻璃盖玻片的体外培养海马神经元。该16位共聚焦图像以63倍物镜拍摄（分辨率1024×1024像素，像素尺寸92.45 nm），采用双通道序列扫描模式：分别靶向标记PSD95蛋白与Homer蛋白。两种蛋白均表达于谷氨酸能突触后位点，富集于突触后致密区（Post Synaptic Density，PSD）。该样本是开展共定位与关联分析测试的理想模型。Homer通道可通过hk均值聚类（hk-means）分割（参考协议：http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/）推断神经元形态，也可借助小波分割（参考协议：http://icy.bioimageanalysis.org/protocol/rainbow-spot-detector-scale-finder/）检测蛋白簇。该样本图像可结合Icy软件的Easy SODA（Standard Object Distance Analysis，标准对象距离分析）协议（参考协议：http://icy.bioimageanalysis.org/protocol/easy-soda-2-colors-1-image/），实现PSD95与Homer蛋白簇之间的关联检测。