The effect of slow and rapid H2S production on the levels of LPS-induced proinflammatory mediators and transcription in different human cell cultures. The effect of slow and rapid H2S production on the levels of LPS-induced proinflammatory mediators and transcription in different human cell cultures

In this study we investigated the effect of two different agents producing hydrogen sulfide (H2S) on LPS-induced inflammatory mediators such as TNFa, NO and ROS. The agents (GYY4137 and sodium hydrosulfide) applied differ significantly by the kinetics of H2S release. In the investigation we compared the effects of H2S release on the LPS-induced inflammation exploring SH-SY5Y human neuroblastoma cell line and human promonocytic cell line, THP-1. Our data clearly show that both hydrogen sulfide producing agents significantly reduced the production of the investigated proinflammatory mediators when applied both before and after LPS administration. Furthermore, transcriptomic studies demonstrated that H2S release ameliorates the expression of multiple proinflammatory genes up-regulated due to LPS administration. However, the pattern of changes observed in transcriptome differs dramatically depending on the time of H2S release (before or after LPS-challenge). Overall design: This series contains data only for THP1 cells. Cells were treated with GYY4137 before or after LPS. Additional cells were treated only with GYY4137 or LPS or were untreated.

本研究探讨了两种产硫化氢 (hydrogen sulfide, H₂S) 制剂对脂多糖 (lipopolysaccharide, LPS) 诱导的炎症介质——肿瘤坏死因子α (tumor necrosis factor α, TNF-α)、一氧化氮 (nitric oxide, NO) 与活性氧 (reactive oxygen species, ROS)——的调控效应。所用制剂分别为GYY4137与硫氢化钠 (sodium hydrosulfide)，二者的硫化氢释放动力学存在显著差异。本研究采用SH-SY5Y人神经母细胞瘤细胞系 (SH-SY5Y human neuroblastoma cell line) 与THP-1人原单核细胞系 (THP-1 human promonocytic cell line) 作为实验模型，对比了硫化氢释放对脂多糖诱导炎症反应的影响。我们的实验数据清晰显示，两种产硫化氢制剂在脂多糖给药前或给药后施加时，均可显著降低所检测的促炎介质的生成量。此外，转录组学 (transcriptomics) 研究证实，硫化氢释放可缓解脂多糖给药后上调表达的多种促炎基因的转录水平。但转录组中观察到的基因表达变化模式，会因硫化氢释放时机——脂多糖刺激前或刺激后——的不同而呈现显著差异。实验整体设计：本数据集仅包含THP-1细胞的相关数据。实验分组包括：脂多糖给药前或给药后施加GYY4137处理的细胞；仅施加GYY4137处理组、仅施加脂多糖处理组以及未做任何处理的空白对照组。