Substrate Stiffness Modulates the Growth, Phenotype, and Chemoresistance of Ovarian Cancer Cells

Purpose: Identify the effect of substrate stiffness on gene expression Methods:Evaluating for differentially expressed mRNAs in the SKOV-3 cells grown on the different substrates via High-throughput sequence Results: We found that the general direction of changes in gene expression of cells grown on the different substrates and the most significant signalling pathways and the expression of gene orthologs broadly involved in platinum drug resistance, apoptosis, cell cycle. Conclusions: Our study represents the first detailed analysis of the effects of substrate stiffness on gene expression of ovarian cancer cells. Overall design: SKOV-3 cells were seeded in the petri dishes, coated with hydrogel substrates of different stiffness (0.5kPa, 4kPa, 25kPa).

【研究目的】明确基质刚度对基因表达的调控效应。
【实验方法】采用高通量测序（High-throughput sequencing）技术，对培养于不同刚度基质上的SKOV-3细胞内的差异表达mRNA进行分析与鉴定。
【实验结果】本研究发现，培养于不同刚度基质的细胞，其基因表达变化的整体趋势，以及与铂类药物耐药、细胞凋亡、细胞周期广泛相关的最显著信号通路和基因直系同源物的表达水平均存在显著差异。
【研究结论】本研究首次详细分析了基质刚度对卵巢癌细胞基因表达的影响。
【实验整体设计】将SKOV-3细胞接种于包被有不同刚度（0.5kPa、4kPa、25kPa）水凝胶基质的培养皿中。