Genome-wide maps of Set1 and H3K4me3 in budding yeast

We report the application of ChIP-sequencing technology for high-throughput profiling of histone modifications in budding yeast. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide maps of Set1 and H3K4me3 in yeast cells. We find that Set1 and H3 lysine 4 trimethylation locate primarily in open reading frame of genes. In addition, we focus on the distribution of Set1 and H3K4me3 in histone gene clusters and found strong similar binding of Set1 and H3K4me3 on all histone genes. Examination of Set1-FLAG, H3 and H3K4me3 in yeast cells. Untagged Set1 was used as control.

本研究报道了染色质免疫共沉淀测序（ChIP-sequencing）技术在酿酒酵母组蛋白修饰高通量谱型分析中的应用。通过对染色质免疫沉淀所得DNA进行测序，我们获得了超过40亿碱基的序列数据，进而绘制了酿酒酵母细胞中Set1与组蛋白H3赖氨酸4三甲基化（H3K4me3）的全基因组图谱。研究发现，Set1与H3K4me3主要定位于基因的开放阅读框（open reading frame, ORF）区域。此外，本研究聚焦于Set1与H3K4me3在组蛋白基因簇中的分布特征，发现二者在所有组蛋白基因上均呈现高度相似的结合模式。本研究对酿酒酵母细胞内的FLAG标记Set1（Set1-FLAG）、组蛋白H3（H3）及H3K4me3开展了检测，并以未标记Set1作为对照。