Impact of Tet inhibition by Bobcat339 on gene expression of porcine parthenogenetic embryos

To explore the effects of Tet on porcine pre-implantation embryogenesis, we utilized Bobcat339, a specific small-molecule inhibitor of the Tet protein, to treat parthenogenetic 4-cell stage embryos. Overall design: Comparative gene expression profiling analysis of RNA-seq data for control and 50 µM Bobcat339 4-cell embryos.

为探究Tet蛋白对猪植入前胚胎发育的影响，本研究采用Tet蛋白特异性小分子抑制剂Bobcat339处理孤雌生殖4细胞期胚胎。实验整体设计：对对照组与50 μM Bobcat339处理组的4细胞期胚胎进行RNA测序（RNA-seq）数据的比较基因表达谱分析。