Zinc pyrithione treatment with 12.6 uM for 2 h. Saccharomyces cerevisiae

The number and type of synthetic chemicals that are being produced worldwide continues to increase significantly. While these industrial chemicals provide numerous benefits, there is no doubt that some have potential to damage the environment and health. Toxicity must be evaluated and use must be carefully controlled and monitored in order to minimize potential damage. DNA microarray technology has become an important new technique in toxicology. We are using the yeast Saccharomyces cerevisiae as a model organism for toxicological study because it is a simple, fast-growing eukaryote that has been thoroughly characterized. In order to evaluate toxicity by newly synthesized or mixture chemicals, toxicity-induced gene expression alteration profiles by known chemicals should be collected. In our study, cells need to be exposed with same experimental cellular condition, semi lethal (IC50), respectively. In the case of zinc pyrithione (CAS; 13463-41-7), the exposure dose was decided as 12.6 uM by growth curve with continuously diluted exposure. Zinc pyrithione is best known for its use in treating dandruff and seborrheic dermatitis. It also has antibacterial properties and is effective against many pathogens from the streptococcus and staphylococcus class. This substrate is suspected to be neurotoxicant and skin or sense organ toxicant. Keywords: stress response Overall design: Series containes 3 hybridization results from independent biological samples, and each experiment have high and low power scanned data respectively.

全球范围内合成化学品的数量与种类仍在显著增长。这类工业化学品虽带来诸多益处，但毋庸置疑，其中部分物质存在破坏环境与损害人体健康的潜在风险。因此，必须对其毒性进行评估，并对使用过程加以严格管控与监测，以尽可能降低潜在危害。DNA微阵列（DNA microarray）技术已成为毒理学领域一项重要的新兴技术。本研究以酿酒酵母（Saccharomyces cerevisiae）作为毒理学研究的模式生物，因其是一种结构简单、生长迅速的真核生物，且已被充分研究表征。为评估新合成化学品或混合化学品的毒性，需先收集已知化学品所诱导的毒性相关基因表达变化谱。本研究中，细胞需在统一的实验培养条件下，分别以半致死（半数抑制浓度，IC50）剂量进行暴露处理。以吡啶硫酮锌（CAS号：13463-41-7）为例，通过连续稀释暴露的生长曲线实验，确定其暴露剂量为12.6 μM。吡啶硫酮锌最广为人知的用途是治疗头皮屑与脂溢性皮炎，同时具备抗菌特性，对链球菌属、葡萄球菌属的多种病原体均有效。该物质被怀疑具有神经毒性，以及皮肤或感觉器官毒性。关键词：应激反应。整体设计：本系列包含3份独立生物学样本的杂交结果，且每份实验分别配有高功率与低功率扫描的数据。