SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in November 2011

Flow cytometry data was collected in November 2011, in waters off South Australia. \n\nThe general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.\n\nSix main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), B4 (35.168S, 135.418E) and B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术（Flow Cytometry）数据采集于2011年11月，采集区域为澳大利亚南部近岸海域。

本研究的核心目标为：厘清澳大利亚南部大陆架海域的生态系统背景信息，阐明上升流与咸水外泄事件对微生物群落的影响，最终构建该区域的生物地球化学模型。

样本采集依托澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目，在研究船Ngerin号（RV Ngerin）的科考航次中完成。

各航次均针对叶绿素荧光最大值（Chlorophyll Fluorescence Maximum, FM）层的物理、化学与生物学特性开展系统调查。

本次采集的流式细胞术数据涵盖超微型浮游植物、细菌与病毒三类研究对象。

本研究共设置6个主要采样站位：其中5个站位位于100米等深线（100 m isobath）上，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、B4（35.168°S，135.418°E）与B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），地处袋鼠岛西南海域。

需说明的是：结合站位间距（14~25海里）、陆架中层海流平均流速分量（0.01 m·s⁻¹，原文原表述为m s21，已修正为标准科学单位格式）与科考船平均航速（约9节）综合分析，各站位所采集的水体团存在显著差异。

研究团队还在部分时段采集了额外样本，点位包括位于袋鼠岛的国家参考站（National Reference Station, NRS，35.832°S，136.447°E），以及发生咸水外泄事件的南澳斯宾塞湾口锚泊站（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）。