TPRI

We downloaded all orthologous sequences of TAa and TPSs of <i>D. stramonium</i>, <i>D. metel</i> and 11 species of Solanaceae to construct the orthogrpups. Downloades sequenceso of proteins and ORF were extracted using TransDecoder and corroborated with GenScan. TPS genes were translated with GenScan, are in the right direction and are the correct CDS; we corroborated this with the NCBI. The database used for BLAST was Uniprot; all available data for the analyzed plant species were downloaded. These amounts 847,544 3. BLAST was carried out with a filtering evalue of 1e-³, and only hits above 85 % were selected. Orthogroups was inferred using Orthofinser v 2.3.3. DIAMONS (Emms and Kelly 2015, 2019) while OrthoMCL was used to assess orthologs among species (Chen et al., 2006).

我们下载了曼陀罗（*D. stramonium*）、洋金花（*D. metel*）及11种茄科植物的TAa与TPS同源序列，以构建同源基因簇（orthogroups）。使用TransDecoder提取蛋白质序列与开放阅读框（Open Reading Frame, ORF），并通过GenScan进行验证。TPS基因经GenScan完成翻译，其序列方向正确且为完整的编码序列（Coding Sequence, CDS），我们通过美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）对该结果进行了验证。本次BLAST分析采用的数据库为UniProt，我们下载了所有待分析植物物种的可用数据，共计847,544条。BLAST分析的E值过滤阈值设置为1e-3，仅保留相似度高于85%的比对结果。我们使用Orthofinder v2.3.3推断同源基因簇，同时采用DIAMOND（Emms与Kelly，2015、2019）及OrthoMCL（Chen等，2006）对物种间的同源序列进行评估。