Genome-wide mapping of the transcription factor GABP-alpha binding in human CD34+CD133+ hematopoietic progenitor cells.

We used ChIP-Seq to map GABP-alpha binding sites in human hematopoietic progenitor cells (HPCs). Coupled with functional assays using GABP-alpha deficient mouse model and bioinformatics analysis, we systematically determined a transcriptional module controlled by GABP in HPCs. Examination of the role of GABP in hematopoietic stem cells

我们采用染色质免疫共沉淀测序（ChIP-Seq）技术，绘制了人类造血祖细胞（hematopoietic progenitor cells, HPCs）内GABP-α的结合位点图谱。结合GABP-α缺陷小鼠模型的功能实验与生物信息学分析，我们系统鉴定出造血祖细胞中由GABP调控的转录模块。此外，本研究还考察了GABP在造血干细胞中的作用。