Rnr1 levels are reduced in ixr1 after DNA damage.

(A) Western blot analysis of Rnr1 levels in wild-type (wt) (W1588-4C), ixr1-S366F (TOY734), and ixr1Δ (TOY736) strains before and after 2 hours treatment with 0.2 mg/L 4-NQO or 200 mM HU. (B) Quantification of Rnr1 levels in wild-type (wt) (W1588-4C), ixr1Δ (TOY736) and ixr1-S366F (TOY734) strains before and after 2 hours treatment with 0.2 mg/L 4-NQO or 200 mM HU. Tubulin was used as the internal control (see Materials and methods). Error bars represent standard error of the mean (SEM). (C) Lower panel, dynamics of Rnr1 decrease and Rnr4 increase in wt (W1588-4C, lanes marked with “w”) and ixr1Δ (TOY736, lanes marked with “i”) strains during a 12-hour time-course incubation with 0.2 mg/L 4-NQO. Upper panel, the corresponding flow-cytometric histograms. (D) Proliferation curves for the experiment in Figure 4C.(A) 野生型（wt，W1588-4C）、ixr1-S366F（TOY734）与ixr1Δ（TOY736）菌株经0.2 mg/L 4-NQO或200 mM HU处理2小时前后的Rnr1蛋白水平蛋白质印迹（Western blot）分析。(B) 野生型（wt，W1588-4C）、ixr1Δ（TOY736）与ixr1-S366F（TOY734）菌株经0.2 mg/L 4-NQO或200 mM HU处理2小时前后的Rnr1蛋白水平定量分析。以微管蛋白（Tubulin）作为内参（详见材料与方法）。误差棒表示平均值标准误（SEM）。(C) 下方泳道图：野生型（W1588-4C，泳道标注"w"）与ixr1Δ（TOY736，泳道标注"i"）菌株经0.2 mg/L 4-NQO孵育12小时的时间进程中，Rnr1蛋白水平下调与Rnr4蛋白水平上调的动态变化。上方泳道图为对应的流式细胞术直方图（flow-cytometric histograms）。(D) 对应图4C实验的增殖曲线。