Canonical ankyrin comparison

Nasonia DV ankyrin domain proteins were blasted to different insect species to search for orthologs. Results of these are given here, and show that the wasp proteins are lineage restrictedfile descriptions:Additional File 1. (.xlsx) D.melanogaster and A.mellifera BLASTp hits for each Nasonia novel ankyrin-repeat containing transcript. Each of the 15 novel Nasonia transcripts was entered into a BLASTp query to search for homologous proteins in Drosophila and Apis. The top 100 hits were collected (duplicate sequences were removed) for each Nasonia transcript (individual sheets within workbook). Each row represents a distinct BLASTp hit and contains the hit’s description (column A), BLAST e-value (column B), and NCBI accession number (column C). <br>Additional File 2. (.xlsx) Top D.melanogaster and A.mellifera BLASTp novel ankyrin-repeat hits. All sequences from Additional file 1 were pooled (duplicate sequences removed) and entered as a reciprocal BLASTp query to see if they were strongly homologous to the Nasonia novel ankyrin-repeat containing transcripts. Each row represents a distinct BLASTp hit from Additioanl file 1, and again contains the hit’s description (column A, Yellow highlights Apis sequences. Drosophila sequences are left in white), BLAST e-value (column B), and NCBI accession number (column C) as well as the description of the Nasonia protein it reciprocal BLASTs back to (column E), BLAST e-value (column F), and NCBI accession number (column G). <br>

本数据集针对金小蜂（Nasonia）DV品系的锚蛋白结构域蛋白，将其与不同昆虫物种进行BLASTp比对以检索直系同源基因，相关分析结果如下，结果显示该寄生蜂蛋白具有谱系限制性。
文件说明：
附加文件1（.xlsx格式）：针对每条金小蜂新型锚蛋白重复序列转录本，在黑腹果蝇（Drosophila melanogaster）与西方蜜蜂（Apis mellifera）中得到的BLASTp比对命中结果。
研究将15条金小蜂新型转录本分别作为BLASTp查询序列，在果蝇与蜜蜂物种中检索同源蛋白。针对每条金小蜂转录本（对应工作簿内的独立工作表），收集排名前100的比对命中结果并去除重复序列。工作表中每一行代表一条独立的BLASTp命中结果，包含以下字段：命中结果描述（A列）、BLAST比对e值（B列）以及NCBI登录号（C列）。
附加文件2（.xlsx格式）：黑腹果蝇与西方蜜蜂中针对金小蜂新型锚蛋白重复序列的顶级BLASTp比对命中结果。
将附加文件1中的所有序列去重后合并，作为反向BLASTp查询序列，以验证其是否与金小蜂新型锚蛋白重复序列转录本存在强同源性。工作表中每一行代表附加文件1中的一条独立BLASTp命中结果，同样包含以下字段：命中结果描述（A列，黄色高亮标注为蜜蜂序列，果蝇序列保留白色背景）、BLAST比对e值（B列）以及NCBI登录号（C列），同时还包含其反向BLAST比对对应的金小蜂蛋白描述（E列）、反向BLAST的e值（F列）以及NCBI登录号（G列）。