Mytilus galloprovincialis hemocytes transcriptome in vitro

Mediterranean mussel is an important aquacultured species which live in a close contact with numerous marine microorganisms. As all invertebrates, they lack an adaptive immune response but it has been reported that invertebrates may respond to an infection involving a high degree of pathogen specificity and innate immune memory or “priming”.In this work we conducted Illumina sequencing of the mussel hemocytes transcriptome stimulated in vitro: triplicates of pools of 25 individuals after 8 hours of stimulation. About 70 million raw reads were obtained from each sample, which were assembled into more than 200,000 contigs.the goal of this experiment is to provide new insights into the innate immune memory or priming in Mytilus galloprovincialis, and its possible aplication in aquaculture.

地中海贻贝（Mediterranean mussel）是一类重要的水产养殖物种，其生存环境与大量海洋微生物密切接触。与所有无脊椎动物一样，该物种缺乏适应性免疫应答，但既往研究显示，无脊椎动物可针对感染产生具有高度病原体特异性的免疫应答，并伴随先天免疫记忆或免疫致敏（priming）现象。本研究对体外刺激后的贻贝血细胞转录组开展了Illumina测序：将25只个体的血细胞混合为样本，设置3次生物学重复，刺激时长为8小时。每个样本可获得约7000万条原始读数（raw reads），经组装后得到超过20万个重叠群（contigs）。本实验的研究目标是为深入理解地中海贻贝（Mytilus galloprovincialis）的先天免疫记忆或免疫致敏现象，及其在水产养殖中的潜在应用价值提供新的研究视角。