Systematic annotation of orphan RNAs reveals blood-accessible molecular barcodes of cancer identity and cancer-emergent oncogenic drivers

The purpose of this experiment is to identify cellular processes that are associated with cellular expression of oncRNAs, which are cancer-emergent non-coding, small RNAs. To test this, we compared the transcriptomes of our breast cancer cells lines (MDA-MB-231 and HCC-LM2) overexpressing oncRNA.ch7.29 or oncRNA.ch17.67 relative to respective controls. Cell lines were transduced with shctrl, oncRNA.ch7.29, or oncRNA.ch17.67 under the control of a U6 promoter. RNA was extracted from the cell lines and used as input for mRNA-seq library construction.

本实验旨在筛选与癌RNA（oncRNAs）的细胞表达相关的细胞生物学过程，其中癌RNA是一类具有致癌性的非编码小RNA。为验证该研究目标，我们将过表达oncRNA.ch7.29或oncRNA.ch17.67的乳腺癌细胞系（MDA-MB-231与HCC-LM2）的转录组，与各自对应的对照组进行了比较分析。所有细胞系均通过U6启动子调控的shctrl、oncRNA.ch7.29或oncRNA.ch17.67进行转导。随后我们从细胞系中提取RNA，将其作为起始原料用于mRNA测序（mRNA-seq）文库的构建。