Data underlying the publication: Linking Metastatic Potential and Viscoelastic Properties of Breast Cancer Spheroids via Dynamic Compression and Relaxation in Microfluidics

The research objective is to investigate the relationship between the viscoelastic properties of breast cancer spheroids and their metastatic potential. The study employs a microfluidic device to subject spheroids to controlled dynamic compression, followed by monitoring their relaxation behavior. The experiments were performed on a brightfield microscope, while the immunostaining images were taken on a confocal microscope. The images were extracted as .tif files and implemented in custom made MATLAB codes to analyse the spheroid compression and relaxation dynamics. Parameters like axial strain (for compression), deformation parameters (for relaxation), circularity were defined and fitted to physical models to extract viscoelastic parameters and relaxation timescales. The file include also the codes to generate the final figures included in the manuscript. A README file helps navigating the folders.<br>

本研究的目标为探究乳腺癌球体（breast cancer spheroids）的粘弹性特性与其转移潜能之间的关联。
本研究采用微流控装置（microfluidic device）对乳腺癌球体施加可控动态压缩，随后监测其松弛行为。
实验在明场显微镜（brightfield microscope）下完成，而免疫染色图像则通过共聚焦显微镜（confocal microscope）采集。
所有图像以.tif格式文件导出，并通过自主编写的MATLAB代码开展球体压缩与松弛动力学过程的分析。
研究定义了轴向应变（axial strain，用于压缩过程分析）、松弛过程的形变参数、圆形度（circularity）等参数，并将这些参数拟合至物理模型，以提取粘弹性参数与松弛时间尺度（relaxation timescales）。
本数据集还包含用于生成论文手稿中最终配图的代码，附带的README文件可帮助用户快速浏览各文件夹结构。