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Raw sequence reads for single amplified genomes (SAGs) of environmental DNA isolated by microencapsulation of coastal seawater

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Figshare2026-03-10 更新2026-04-28 收录
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https://figshare.com/articles/dataset/Raw_sequence_reads_for_single_amplified_genomes_SAGs_of_environmental_DNA_isolated_by_microencapsulation_of_coastal_seawater/31591414
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Reads used to generate single-amplified genomes (SAGs) from DNA in microencapsulated coastal seawater. These Illumina reads were [1] initially demultiplexed with bcl2fastq (yielding a set of reads for each Illumina library), then [2] secondarily demultiplexed with Pheniqs (ideally yielding a set of reads for each microcapsule, based on the likelihood that each microcapsule had a distinct random combination of Atrandi barcodes), before [3] Atrandi barcodes were removed with TrimGalore, leaving paired end reads that were [4] quality-trimmed with Trimmomatic, then finally [5] interleaved. These reads are "raw" in only the broadest sense, i.e. in that no decontamination or normalization has been performed. See publication for method details: https://doi.org/10.1038/s41564-025-02167-5These zipfiles include reads from 2,037 SAGs, which have been zipped together in 5 batches (of ~5 GB each) for ease of transfer.
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2026-03-10
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