Raw sequence reads for single amplified genomes (SAGs) of environmental DNA isolated by microencapsulation of coastal seawater

Reads used to generate single-amplified genomes (SAGs) from DNA in microencapsulated coastal seawater. These Illumina reads were [1] initially demultiplexed with bcl2fastq (yielding a set of reads for each Illumina library), then [2] secondarily demultiplexed with Pheniqs (ideally yielding a set of reads for each microcapsule, based on the likelihood that each microcapsule had a distinct random combination of Atrandi barcodes), before [3] Atrandi barcodes were removed with TrimGalore, leaving paired end reads that were [4] quality-trimmed with Trimmomatic, then finally [5] interleaved. These reads are "raw" in only the broadest sense, i.e. in that no decontamination or normalization has been performed. See publication for method details: https://doi.org/10.1038/s41564-025-02167-5These zipfiles include reads from 2,037 SAGs, which have been zipped together in 5 batches (of ~5 GB each) for ease of transfer.本数据集的测序读段用于从微包被的海岸海水中的DNA生成单扩增基因组（single-amplified genomes, SAGs）。这批Illumina测序读段的预处理流程如下：[1] 首先通过bcl2fastq完成初次解复用，为每个Illumina文库生成对应读段集合；[2] 随后使用Pheniqs进行二次解复用——基于各微包被携带独特Atrandi条码组合的概率，为每个微包被生成对应读段集合；[3] 再通过TrimGalore移除Atrandi条码，得到双端读段；[4] 继而使用Trimmomatic对读段进行质量修剪；[5] 最终将修剪后的读段整理为交错排列格式。本数据集所指的"原始"仅为宽泛意义上的原始，即未进行任何去污染或数据标准化处理。详细实验方法参见发表文献：https://doi.org/10.1038/s41564-025-02167-5。本数据集的压缩包包含2037个SAGs对应的测序读段，为便于传输，已将其打包为5个批次（每个批次约5 GB）。