Total proteome analysis of SK-N-BE2 cells infected with VZV for 48 hours

To understand the molecular features associated with VZV infection in humans, we applied an orthogonal proteomic approach that assayed the interactions between VZV and its host in the neuroblastoma SK-N-BE2 cell line. First, we infected SK-N-BE2 cells with VZV rOka for 48 hours and analysed the induced proteome changes by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).

为解析与人类水痘带状疱疹病毒（Varicella-Zoster Virus, VZV）感染相关的分子特征，本研究采用正交蛋白质组学方法，对神经母细胞瘤SK-N-BE2细胞系中VZV与其宿主的相互作用进行了检测。首先，我们使用VZV rOka毒株感染SK-N-BE2细胞48小时，并通过液相色谱-串联质谱法（LC-MS/MS）分析了感染诱导的蛋白质组变化。