DICER-LIKE1a autoregulation is required for stress adaptation in Physcomitrella patens

In Physcomitrella patens DICER-LIKE1a (PpDCL1a) encoding the essential Dicer protein for microRNA biogenesis harbors an intronic microRNA (miR1047) suggesting an autoregulatory mechanism controlling PpDCL1a abundance. In this project we have analyzed the effects of abolished autoregulation of the PpDCL1a transcript at miRNAs and mRNAs levels at both normal and salt stress conditions.

在小立碗藓（Physcomitrella patens）中，编码微小RNA（microRNA）生物发生所必需的Dicer蛋白的DICER-LIKE1a（PpDCL1a）基因，携带有一个内含子微小RNA（intronic microRNA，miR1047），这提示存在一种调控PpDCL1a表达丰度的自调控机制。本研究分析了在正常及盐胁迫条件下，PpDCL1a转录本的自调控被消除后，对微小RNA（miRNA）和信使RNA（mRNA）水平的影响。