Data and code for: High fidelity fibre-based physiological sensing deep in tissue

Physiological sensing deep in tissue, remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 µm diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (± 0.02 pH units and ± 0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.

组织深部的生理传感仍是临床领域的一大挑战。本文报道了一款柔性微型传感光极（Optrode），可为微创体内原位测量提供技术平台。将共价偶联高密度比率型荧光团的二氧化硅微球，沉积于直径150 µm的多芯光纤远端的刻蚀凹槽中。基于该平台，本研究实现了肺远端肺泡腔内高精度的pH与氧浓度光子学测量。本研究证实了如下现象：共价偶联于二氧化硅微球的羧基荧光素经高密度沉积后，其荧光会随pH变化呈现反向偏移。该平台可实现快速精准的测量，精度可达±0.02 pH单位、±0.6 mg/L氧浓度，响应时间接近瞬时，且架构灵活，可拓展集成多传感器。