IV.3

Somatic mutations accumulated in fibroblast colony during ~40 passages.Fibroblasts obtained from individual IV.3. Mutations in the colony at the end time point of the experiment were called against colonies at the start time point of the experiment using Mutect2.在约40次传代过程中，成纤维细胞（fibroblast）集落内积累了体细胞突变（somatic mutation）。实验所用成纤维细胞取自个体IV.3。本研究使用Mutect2工具，以实验起始时点的细胞集落为参照，对实验结束时点的细胞集落中的突变进行变异检出。