Data for manuscript entitled " Metabolism fine tuning and cardiokines secretion represent adaptative responses of the heart to High Fat and High Sugar Diets in flies"
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This repository contains Data and Raw images used for the analysis and the graphical representation used in the 6 figures and 6 supplemental figures of our manuscript. Each file contains compressed data corresponding to one figure and its associated supplemental figure. Figure 1_Figure S1: scaled data (.csv) of cardiac imaging controlKK_60100 controlGD_60000 controlGFP Figure 2_Figure S2: rnaseq report genes lists for heatmap Figure 2E,F r script for heatmap graphical representation Figure 3_Figure S3: contains 5 folders corresponding to each individual candidate gene validated and presented (Gnmt, Sardh, Galk, Snmp& and nebu). For Gnmt, Snmp1, GalK and nebu: sub-folders cardiac imaging: scaled data (.csv) of cardiac imaging images: M-modes and positions lines, captured images For Sardh: scaled data (.csv) of cardiac imaging Figure 4_Figure S4: scaled data (.csv) of cardiac imaging Figure 5_Figure S5: contains 4 sub-folders corresponding to genotypes presented in the figures' panels HandfitSP, HandfitKK, HandfitWT: scaled data (.csv) of cardiac imaging fit81: scaled data (.csv) of cardiac imaging and captured images RTqPCR Figures 3 and 5 hearts: data results and analysis (.xls) Figure 6_Figure S6: contains 4 sub-folders Food intake: data raw and normalized (.xls) Western blotting: raw images Imaging confocal: HandfitWT_HandfitKK_ starvation refeeding: raw images and projections, data analysis HandfitOE ND HSD HFD: raw images and projections, data analysis Hand FitKK ND: raw images and projections, data analysis
本仓库包含用于本论文手稿中6幅主图及6幅补充图的分析与可视化绘图所用的数据与原始图像。每个文件对应一幅主图及其配套补充图的压缩数据。 图1_图S1：心脏成像标准化数据（.csv），包含样本分组：controlKK_60100、controlGD_60000、controlGFP。 图2_图S2：包含RNA测序（RNA-seq）分析报告、热图绘制所需基因列表（对应图2E、F），以及热图可视化的R脚本。 图3_图S3：包含5个文件夹，分别对应经验证并展示的各候选基因（Gnmt、Sardh、Galk、Snmp1及nebu）。 其中针对Gnmt、Snmp1、GalK与nebu的子文件夹包含： - 心脏成像标准化数据（.csv） - 图像文件：M型超声图像及定位线、实拍图像 针对Sardh的子文件夹包含：心脏成像标准化数据（.csv）。 图4_图S4：心脏成像标准化数据（.csv）。 图5_图S5：包含4个子文件夹，对应图中展示的各基因型： - HandfitSP、HandfitKK、HandfitWT：心脏成像标准化数据（.csv） - fit81：心脏成像标准化数据（.csv）及实拍图像 对应图3与图5心脏样本的实时定量聚合酶链反应（RT-qPCR）实验：数据结果与分析文件（.xls）。 图6_图S6：包含4个子文件夹： 1. 食物摄入实验：原始数据及标准化数据（.xls） 2. 蛋白质免疫印迹（Western blotting）：原始图像 3. 共聚焦成像： 4. 分组干预实验： - HandfitWT_HandfitKK_饥饿-复喂养模型：原始图像及投影结果、数据分析文件 - HandfitOE ND HSD HFD：原始图像及投影结果、数据分析文件 - HandfitKK ND：原始图像及投影结果、数据分析文件
