Single-cell sequencing of colorectal tumors in mice

To explore the underlying mechanism of STK25 in CRC, we performed single-cell transcriptome profiling of the intestinal tumor from STK25 wKO and wile-type mice.We performed dimensional reduction and unsupervised clustering of cells to identify cell groups based on their expression patterns Intestinal tumor from STK25 wKO and wile-type mice were isolated by Fluorescence-activated cell sorting(FACS) according to the presence or absence of mCherry signal and analyzed by using scRNAseq.为探究丝氨酸/苏氨酸激酶25（STK25）在结直肠癌（CRC）中的潜在作用机制，我们对STK25敲除（wKO）及野生型（wild-type）小鼠的肠道肿瘤开展了单细胞转录组测序分析。我们基于细胞的基因表达模式，对细胞进行降维与无监督聚类分析，以鉴定不同的细胞类群。我们根据mCherry信号的有无，通过荧光激活细胞分选术（Fluorescence-activated cell sorting, FACS）分离得到STK25敲除与野生型小鼠的肠道肿瘤细胞，并采用单细胞RNA测序（scRNAseq）完成后续分析。