The effects of chemokines CCL2/7 on MDA-MB-231-FOXC1 cells

Our preliminary studies performed in vitro show that MDA-MB-231-FOXC1 cell, which were used to mimic the lung-colonizing triple-negative breast cancer cells, was an indispensable component for the induction of migration and tube formation of lung endothelial cells. Moreover, our results further show that mouse lung fibroblast-derived chemokines CCL2/7 act on MDA-MB-231-FOXC1 cells, which mediates the migration and tube formation of lung endothelial cells in vitro. To understand the signaling pathways activated by CCL2/7 in MDA-MB-231-FOXC1 cells, we performed RNA-Seq assay. Overall design: MDA-MB-231-FOXC1 cells were treated with recombinant mouse Ccl2 (10 nM)/7 (50 nM) for 6 hours.本研究的体外初步实验结果显示，用于模拟定植于肺部的三阴性乳腺癌细胞的MDA-MB-231-FOXC1细胞，是诱导肺内皮细胞迁移及管腔形成的不可或缺的组成部分。 此外，本研究结果进一步证实，小鼠肺成纤维细胞衍生的趋化因子CCL2/7可作用于MDA-MB-231-FOXC1细胞，进而介导体外肺内皮细胞的迁移与管腔形成。 为探究CCL2/7在MDA-MB-231-FOXC1细胞中激活的信号通路，本研究开展了RNA测序（RNA-Seq）检测。 整体实验设计：将MDA-MB-231-FOXC1细胞用重组小鼠Ccl2（10 nM）/Ccl7（50 nM）处理6小时。