Genome wide maps of ELK1 bingding sites in HDFs control and mechanic loading groups.. Genome wide maps of ELK1 bingding sites in HDFs control and mechanic loading groups.

HDFs were cultured for 24 hrs for adhesion and to reach 50-70% confluence before mechanical tension was applied. Cyclic mechanical stretch (CMS) with a 0.5-Hz sinusoidal curve at 10-20% elongation was applied using a Flexcell○RFX-5000TM Tension System during the stretching. Cells were harvested immediately after the application of CMS stimulation was completed. Control cells were cultured on the same plates in the same incubator with no stretching. Overall design: Examination of ELK1's different bind motifs of HDFs in the control condition and mechanic loading condition.

将人真皮成纤维细胞（HDFs）培养24小时以实现贴壁，并使细胞汇合度达到50%~70%，随后施加机械张力。使用Flexcell®FX-5000™张力系统，施加频率为0.5Hz的正弦曲线型周期性机械拉伸（Cyclic mechanical stretch, CMS），拉伸幅度为10%~20%伸长率。待CMS刺激结束后，立即收集细胞。对照组细胞于相同培养板、相同培养箱中培养，未施加任何拉伸刺激。整体实验设计：探究对照条件与机械加载条件下HDFs中ELK1的不同结合基序。