FTIR Analysis Data.

This study investigates the bioremediation potential of bacterial isolates from diesel-contaminated soils in Dhaka, Bangladesh. A total of 34 morphologically distinct bacterial strains were isolated from hydrocarbon-polluted sites, with Acinetobacter baumannii emerging as the dominant species (41.2%), followed by Pseudomonas otitidis and Klebsiella pneumoniae (11.8% each). Genetic screening revealed that 32.35% of isolates harbored the alkB gene (alkane hydroxylase), while 58.82% carried catE (catechol-2,3-dioxygenase), indicating a strong predisposition for aromatic hydrocarbon degradation. Following turbidimetric screening of 34 bacterial isolates, 11 demonstrating superior growth were selected for gravimetric degradation assessment. Among these, isolate MB 1002 (Pseudomonas nitroreducens) demonstrated the highest degradative capability at 46.92%, followed by MB 751 (Acinetobacter baumannii) at 41.18% and MB 750 (Pseudomonas aeruginosa) at 39.16%. The MB 1002 and MB 751 both were positive for alkB. FTIR analysis revealed that both MB 751 and MB 1002 contribute to diesel degradation, with MB 751 showing stronger oxidation patterns due to the presence of carboxyl functional groups. Due to its superior oxidative capability, MB 751 (designated A. baumannii DUEMBL6) was selected for whole-genome sequencing. The sequence analysis of A. baumannii DUEMBL6 (deposited as JBLODW000000000) revealed: (1) hydrocarbon degradation genes (alkB, ssuD, catechol dioxygenases); (2) 7 biosynthetic gene clusters including siderophores (100% similarity to baumannoferrin); and (3) complete xenobiotic degradation pathways for aliphatic/aromatic compounds. Despite its bioremediation potential, A. baumannii DUEMBL6 harbored 26 antibiotic resistance genes (e.g., blaOXA-338, adeABC efflux pumps) and 33 virulence factors (e.g., csu pilus, biofilm genes), with an 86.1% pathogenicity probability. These findings highlight A. baumannii DUEMBL6’s dual role as a promising bioremediation agent and a potential public health risk, necessitating careful strain selection for environmental applications.本研究针对孟加拉国达卡地区柴油污染土壤中的细菌分离株，探究其生物修复潜力。研究从烃类污染位点中共分离得到34株形态学特征各异的细菌菌株，其中鲍曼不动杆菌（Acinetobacter baumannii）为优势菌种，占比达41.2%；其次为耳假单胞菌（Pseudomonas otitidis）与肺炎克雷伯菌（Klebsiella pneumoniae），二者占比均为11.8%。遗传筛选结果显示，32.35%的分离株携带alkB基因（alkane hydroxylase，烷烃羟化酶），58.82%的分离株携带catE基因（catechol-2,3-dioxygenase，儿茶酚-2,3-双加氧酶），表明该类菌株具备较强的芳香烃降解倾向。通过比浊法对34株细菌分离株进行初筛后，选取11株生长性能优异的菌株开展重量法降解性能评估。其中，菌株MB 1002（还原假单胞菌Pseudomonas nitroreducens）的降解能力最强，可达46.92%；紧随其后的是MB 751（鲍曼不动杆菌Acinetobacter baumannii，降解率41.18%）与MB 750（铜绿假单胞菌Pseudomonas aeruginosa，降解率39.16%）。MB 1002与MB 751均为alkB基因阳性菌株。傅里叶变换红外光谱（FTIR）分析表明，MB 751与MB 1002均可介导柴油降解，其中MB 751因存在羧基官能团而展现出更强的氧化活性。鉴于其优异的氧化能力，MB 751（命名为A. baumannii DUEMBL6）被选为全基因组测序对象。对A. baumannii DUEMBL6（基因序列提交号为JBLODW000000000）的序列分析结果如下：(1) 烃类降解基因（alkB、ssuD、儿茶酚双加氧酶）；(2) 7个生物合成基因簇，其中包含与baumannoferrin相似度达100%的铁载体合成簇；(3) 完整的脂肪族与芳香族化合物外源物降解通路。尽管具备可观的生物修复潜力，A. baumannii DUEMBL6携带26个抗生素耐药基因（如blaOXA-338、adeABC外排泵）与33个毒力因子（如csu菌毛、生物膜相关基因），致病概率为86.1%。本研究结果凸显了A. baumannii DUEMBL6的双重特性：既是极具应用前景的生物修复制剂，同时也可能构成公共卫生风险，因此在环境应用中需谨慎筛选菌株。