A chromatin immunoprecipitation database for prokarytic organisms [Staphylococcus aureus]. A chromatin immunoprecipitation database for prokarytic organisms [Staphylococcus aureus]

Bacterial transcription factors (TFs) regulate gene expression to adapt to changing environments; when combined, the TF’s regulatory actions comprise transcriptional regulatory networks (TRNs). The chromatin immunoprecipitation (ChIP) assay is the major contemporary method for mapping in vivo protein-DNA interactions in the genome. It enables the genome-wide study of transcription factor binding sites (TFBSs) and gene regulation. Here, we present the genome-wide binding for major TFs in Staphylococcus aureus USA300 strains. Overall design: Identification of genome-wide bindings for transcription factors in Staphylococcus aureus using ChIP-exo technology

细菌转录因子（Transcription Factors, TFs）通过调控基因表达以适应不断变化的环境；各类转录因子的调控作用共同构成转录调控网络（Transcriptional Regulatory Networks, TRNs）。染色质免疫共沉淀（Chromatin Immunoprecipitation, ChIP）技术是当前用于绘制基因组内活体蛋白质-DNA互作的主流方法，可实现全基因组范围内转录因子结合位点（Transcription Factor Binding Sites, TFBSs）与基因调控的研究。本数据集提供了金黄色葡萄球菌（Staphylococcus aureus）USA300菌株中主要转录因子的全基因组结合图谱。实验整体设计：采用ChIP-exo技术鉴定金黄色葡萄球菌中转录因子的全基因组结合位点。