Flesh coloration on Atlantic salmon

We compared Illumina TruSeq kit, the standard RNA-Seq and Lexogen QuantSeq kit, the 3 mRNA-Seq method for an Atlantic salmon flesh color investigation. Firstly, QuantSeq was used to compare differentially expressed genes between four different flesh color phenotypes. To confirm the results and to analyze whether there are any differences between TruSeq and QuantSeq, TruSeq was used on two chosen sample subsets from two flesh color phenotypes.

本研究针对大西洋鲑鱼肉色开展相关探究，对比了两款转录组测序试剂盒：Illumina TruSeq试剂盒（Illumina TruSeq kit，配套标准RNA测序（RNA-Seq）流程）与Lexogen QuantSeq试剂盒（Lexogen QuantSeq kit，配套3' mRNA测序方法）。首先，本研究使用Lexogen QuantSeq试剂盒，分析四种不同肉色表型样本间的差异表达基因，以比较不同肉色表型的基因表达差异。为验证上述实验结果，并探究TruSeq与QuantSeq两种测序方法是否存在实验差异，本研究选取源自两种肉色表型的两组样本子集，采用Illumina TruSeq试剂盒完成测序分析。