Data and analysis of diet-induced and obesity-associated alterations of gut microbiota of 129S6/Sv and C57BL/6J mice

It is well known that the microbiota of high fat (HF) diet-induced obese mice differs from that of lean mice, but to what extent this difference reflects the obese state or the diet is unclear. To dissociate changes in the gut microbiota associated with high HF feeding from those associated with obesity, we took advantage of the different susceptibility of C57BL/6JBomTac (BL6) and 129S6/SvEvTac (Sv129) mice to diet-induced obesity and of their different responses to inhibition of cyclooxygenase (COX) activity, where inhibition of COX activity in BL6 mice prevents HF diet-induced obesity, but in Sv129 mice accentuates obesity. <br>Using HiSeq-based whole genome sequencing we identified taxonomic and functional differences in the gut microbiota of the two mouse strains fed regular low fat or HF diets with or without supplementation with the COX-inhibitor, indomethacin. <br> Here we present the sequence assemblies and annotations for those 54 samples, together with the gene catalogue and relevative abundance levels of both genes and OTUs. It is hoped these data can be used for comparison in future studies of a similar design.

众所周知，高脂（high fat, HF）饮食诱导肥胖小鼠的肠道菌群与瘦小鼠存在显著差异，但目前尚不清楚这种差异在多大程度上源于肥胖状态，又在多大程度上由饮食结构所导致。<br>为了厘清高脂饮食相关与肥胖相关的肠道菌群变化之间的差异，我们利用了C57BL/6JBomTac（BL6）与129S6/SvEvTac（Sv129）小鼠对饮食诱导肥胖的易感性差异，以及二者对环氧合酶（cyclooxygenase, COX）活性抑制的不同响应：BL6小鼠经COX活性抑制后可阻断高脂饮食诱导的肥胖，而Sv129小鼠经相同处理则会加剧肥胖。<br>本研究采用基于HiSeq平台的全基因组测序技术，对喂食普通低脂饮食、高脂饮食，或联合补充COX抑制剂吲哚美辛（indomethacin）的两种小鼠品系的肠道菌群进行分析，鉴定出二者在分类学与功能学层面的菌群差异。<br>本文公开了上述54个样本的序列组装结果与注释信息，同时附带基因目录以及基因与操作分类单元（operational taxonomic unit, OTU）的相对丰度数据。<br>期望本数据集可为后续同类设计的相关研究提供对比参考依据。