Expression data from the hearts of a mouse model of spontaneous myocardial infarction. Mus musculus

Mice with homozygous null mutations in the HDL receptor (SR-BI) and apoE genes (SR-BI KO/apoE double KO (dKO) mice) spontaneously develop occlusive, atherosclerotic coronary artery disease (CAD) and die prematurely (50% mortality at 42 days of age) on standard chow diet feeding. Microarray analysis was performed to investigate the changes in gene expression profiles during the development of spontaneous severe CAD, which includes myocardial infarction and heart failure. These data will provide new insights in understanding the pathophysiology of CAD. Overall design: The whole Hearts from dKO or SR-BI+/- apoE-/- (HET) mice (n=9-12) were harvested at 21, 31 and 43 days of age, and analyzed using Affymetrix microarrays. dKO mice do not show detectable signs of CAD at 21 days of age, small myocardial infarction (MI) and heart failure at 31 days of age, and extensive MI and severe heart failure at 43 days of age (50% mortality at 42 days of age). Each mouse was assigned to one array. SR-BI+/- apoE-/- mice (HET) which do not develop detectable signs of CAD on chow diet were used as controls.The data also include those from probucol treated dKO and HET mice (n=2-8).

高密度脂蛋白（HDL）受体SR-BI与载脂蛋白E（apoE）基因纯合敲除的小鼠（SR-BI敲除/apoE双敲除（dKO）小鼠）在标准普通饲料喂养下，会自发发生闭塞性动脉粥样硬化性冠状动脉疾病（CAD）并过早死亡，42日龄时死亡率达50%。为探究自发重度冠状动脉疾病（涵盖心肌梗死与心力衰竭）发生进程中的基因表达谱变化，本研究开展了微阵列分析。该数据将为阐明冠状动脉疾病的病理生理学机制提供新的见解。

实验整体设计如下：分别于21、31和43日龄采集dKO小鼠或SR-BI杂合子/apoE敲除（HET）小鼠的全心脏样本（每组n=9-12），采用Affymetrix微阵列进行检测分析。dKO小鼠在21日龄时无任何可检测到的冠状动脉疾病迹象，31日龄时出现小型心肌梗死（MI）与心力衰竭，43日龄时出现广泛心肌梗死与重度心力衰竭（42日龄时死亡率达50%）。每只小鼠对应一张微阵列芯片。以普通饲料喂养下未出现可检测冠状动脉疾病迹象的SR-BI+/- apoE-/-（HET）小鼠作为对照。本数据集还包含普罗布考（probucol）处理的dKO与HET小鼠的相关数据（每组n=2-8）。