small-RNA-Seq in human T-cell lymphoblastic lymphoma samples and control thymuses. small-RNA-Seq in human T-cell lymphoblastic lymphoma samples and control thymuses

Precursor T-cell lymphoblastic neoplasms are aggressive haematological neoplasm that most often manifest with extensive marrow and blood affectation (T-cell acute lymphoblastic leukaemia or T-ALL) or less commonly as a thymic mass with limited bone marrow infiltration (T-cell lymphoblastic lymphoma or T-LBL). Here we show data from small-RNA-Seq in a sample series of T-LBL from Spanish patients.The goal was to determine the levels of expression of coding genes and microRNAs, and to identify all genetic variants including SNVs, indels, and fusion transcripts. Overall design: Expression data were determined by comparson of each tumour sample with two control thymuses (404 and 405). Genetic variants were determined by comparison of tumour sequences with canonical ENSEMBL normal-references of each gene.

前驱T细胞淋巴母细胞肿瘤（Precursor T-cell lymphoblastic neoplasms）属于侵袭性血液系统肿瘤，多数病例以广泛的骨髓及血液受累为主要表现，即T细胞急性淋巴细胞白血病（T-cell acute lymphoblastic leukaemia, T-ALL）；少数病例则表现为伴有限度骨髓浸润的胸腺肿物，即T细胞淋巴母细胞淋巴瘤（T-cell lymphoblastic lymphoma, T-LBL）。本研究展示了西班牙患者T-LBL队列样本的小RNA测序（small-RNA-Seq）数据，研究目标为明确编码基因与微小RNA（microRNA）的表达水平，并鉴定所有遗传变异，包括单核苷酸变异（SNVs）、插入缺失（indels）以及融合转录本。整体实验设计：通过将每例肿瘤样本与两例对照胸腺样本（编号404与405）进行比对获取基因表达数据；遗传变异的鉴定则通过将肿瘤测序序列与各基因的标准ENSEMBL正常参考序列进行比对完成。