Genome-wide maps of EWS-FLI1 binding sites and histone modification in murine Ewing sarcoma cells. Genome-wide maps of EWS-FLI1 binding sites and histone modification in murine Ewing sarcoma cells

We identified global DNA binding properties of EWS-FLI1 in mouse Ewing sarcoma. GGAA microsatellites were found as binding sites of EWS-FLI1 but with less frequency than that in human Ewing sarcoma, and genomic distribution is not conserved between human and mouse. Overall design: Examination of binding sites of EWS-FLI1, histone H3K27Ac and H3K27me3 in two Ewing sarcoma cell lines.

我们鉴定了小鼠尤因肉瘤（Ewing sarcoma）中EWS-FLI1的全基因组DNA结合特性。研究发现GGAA微卫星（GGAA microsatellites）可作为EWS-FLI1的结合位点，但其结合频率较人类尤因肉瘤更低，且二者的基因组分布在人与小鼠之间并不保守。实验设计概述：对两种尤因肉瘤细胞系中EWS-FLI1、组蛋白H3K27乙酰化（histone H3K27Ac）以及组蛋白H3K27三甲基化（H3K27me3）的结合位点进行检测。