Transcriptional regulation by the novel Rho GTPase RhoBTB2.. Homo sapiens

RhoBTB2 is a novel Rho GTPase that undergoes loss, underexpression and mutation in breast and lung cancer. We have shown that we can mimic loss of RhoBTB2 through siRNA treatment of primary cells. We propose to perform comparative microarray analysis of primary lung cells to establish the identification of the gene targets of RhoBTb2 regulation. Keywords: Effects of siRNA expression Overall design: Primary lung cells (NHBE) transfected with siRNA oligonucleotides against cyclophilin or Lamin A/C (controls) were compared to cells transfected with siRNA oligonucleotides against RhoBTB2. We used two siRNA oligonucleotides against RhoBTB2 (1 and 2) to determine any non-specific effects. Cells expressed the siRNA oligonucleotides for 72 hours before total RNA extraction. 10 samples have been analysed.

RhoBTB2是一种新型Rho GTP酶（Rho GTPase），在乳腺癌与肺癌中存在缺失、低表达及突变现象。本研究已证实，可通过对原代细胞进行小干扰RNA（siRNA）处理，模拟RhoBTB2的表达缺失。本研究拟对原代肺细胞开展比较微阵列（microarray）分析，以鉴定RhoBTB2调控的靶基因。
关键词：siRNA表达的效应
实验设计概述：将转染了靶向亲环蛋白（cyclophilin）或核纤层蛋白A/C（Lamin A/C）的小干扰RNA寡核苷酸的原代肺细胞（NHBE），与转染了靶向RhoBTB2的小干扰RNA寡核苷酸的原代肺细胞进行对照比较。本研究使用2条靶向RhoBTB2的小干扰RNA寡核苷酸（1号与2号），以排除非特异性效应。细胞在转染并持续表达小干扰RNA寡核苷酸72小时后提取总RNA，共完成10个样本的分析。