Hepatic transcriptome of rats treated with vehicle or fipronil (3 mg/kg/d per os for 14 days)

Fipronil (CAS #: 120068-37-3), a widely used insecticide, has been described as a thyroid disruptor in rat inducing a marked increase in thyroxine (T4) clearance resulting in a decrease in T4 plasma concentration. These effects seem to require the bioactivation of fipronil via its biotransformation into fipronil sulfone by cytochromes P450 (CYP). Here, we hypothesized that fipronil-induced thyroid disruption may, at least in part, result from the induction of hepatic enzymes involved in the metabolism of thyroid hormones. Thus, we used microarrays to perform a genome-wide analysis of the effects of fipronil on gene expression in rat liver. Liver gene expression was measured from female Wistar rats treated with vehicle (n = 7) or fipronil 3 mg/kg/day per os for 14 days (n = 8)

氟虫腈（Fipronil，CAS登记号：120068-37-3）是一种广泛应用的杀虫剂，已有研究表明其可作为大鼠甲状腺干扰物，能够显著提升甲状腺素（T4）的清除率，进而降低血浆中T4的浓度。该类效应似乎需要通过细胞色素P450（cytochromes P450, CYP）将氟虫腈生物转化为氟虫腈砜，以完成氟虫腈的生物活化过程。本研究假设，氟虫腈诱导的甲状腺干扰效应至少部分源于参与甲状腺激素代谢的肝酶的诱导表达。为此，我们采用微阵列（microarrays）技术对氟虫腈作用于大鼠肝脏的基因表达情况开展全基因组分析。实验以雌性Wistar大鼠为受试对象，分别经口给予赋形剂（n=7）或3 mg/kg/天剂量的氟虫腈，连续染毒14天（n=8）