Variant calls in the spike gene from the sequencing of viral cDNA derived from two rVSV-Rs3367-CoV populations.

Analysis of spike sequences derived from long-read sequencing of cDNA isolated from viral populations generated by the plasmid-based rVSV rescue of rVSV-Rs3367-CoV. See Materials and Methods for the analysis workflow. Variant calls are reported in VCF format and were filtered for a quality score (QUAL) ≽ 50. Data from two biological replicates are shown.
(XLSX)

本研究针对通过质粒拯救rVSV-Rs3367-CoV所获得的病毒群体中分离得到的互补DNA（cDNA）进行长读长测序，进而对其刺突序列开展分析。分析流程详见材料与方法部分。变异位点调用结果以VCF格式（Variant Call Format）报告，并经过质量评分（QUAL）≥50的过滤条件筛选。本数据集展示了两份生物学重复的实验数据。(XLSX)