BETA-FOR_SP4_Nematodes_2022

We took four soil samples per patch (5cm diameter, 10 cm depth) within 30 cm radius from TOMST logger after roughly removing the litter. We pooled these samples per patch, sieved the soil (2mm) and extracted nematodes as soon as possible (nematodes need to be alive for this method) with the Baermann-Funnel Method. To do so, we used ~20 g fresh soil. After extraction, nematoes were fixed in 4% formalin, counted and 100 individuals (10% if more than 1000 in the sample) morphologically determined. The dry weight of the soil sample is determined and the number of nematodes of each taxa in 1 g dry weight is calculated.我们在距离TOMST环境记录仪（TOMST logger）30厘米半径范围内的每个样地中，先粗略移除地表枯落物，随后采集4份直径5厘米、深度10厘米的土壤样本。将每个样地的所有土壤样本混匀后，过2毫米孔径筛，随后尽快采用贝尔曼漏斗法（Baermann-Funnel Method）提取线虫——该方法要求线虫保持活体状态，本次提取使用约20克新鲜土壤。提取完成后，将线虫用4%福尔马林溶液固定，进行计数并选取100个个体开展形态学鉴定；若单份样本中线虫总量超过1000，则选取总量10%的个体进行鉴定。最后测定土壤样本的干重，计算每克干土中各分类单元的线虫种群数量。